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. 2020 Apr 14;11:1772. doi: 10.1038/s41467-020-15642-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Principle of operation based on interference between scattered and reflected light combined with ratiometric imaging. b Scatter plot of binding events for NADH:ubiquinone oxidoreductase (respiratory complex I, 12.5 nM), and the corresponding mass histogram. c Negative-stain micrograph of the same sample with individual species corresponding to the peaks in b highlighted. MP images of species with the respective mass are shown for comparison. Scale bars: 50 nm (nsEM) and 200 nm (MP). d Mass distribution for 21 nM trimeric cohesin; upper right, cartoon of trimeric cohesin. e Rotary shadowing EM micrograph of trimeric cohesin shows intrinsic conformational flexibility. Scale bars: 50 nm. f A mixture of two interacting NPC-I and II subcomplexes before (top) and after (bottom) chemical crosslinking with 0.1% glutaraldehyde for 5 minutes followed by quenching. g Reproducibility of the crosslinking procedure shown in f in terms of mole fractions of the three main species before (diamonds) and after crosslinking (rectangles). Source data are provided as a Source Data file.