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. 2020 Apr 14;11:1772. doi: 10.1038/s41467-020-15642-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a SDS-PAGE gels of two-step affinity purification of proteasomes—pull down with GST-Ubl (left) and separation on 10–30% sucrose gradient (right). Individual proteasome subunits are shown in dark red and yellow and PIPs in pink. b Representative negative-stain micrograph and 2D class averages of proteasome complexes generated by nsEM analysis. Scalebar: 50 nm. c MP distributions as a function of NaCl concentration. All reactions were carried out at 4 °C. d Corresponding changes to the abundances of the main species comparing nsEM (triangles) with MP (circles) as well as a breakdown of the main 26S and 30S (dark) and Ecm29-bound (light) species. Error bars are calculated as the standard deviation of mole fractions from different repeats. e MP distributions as a function of different nucleotide conditions. All reactions were carried out at 37 °C. Source data are provided as a Source Data file.