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DiscoverMP analysis parameters for all protein complexes. The values for threshold 2 (0.25) and median filter kernel (15) remained constant for all protein samples.
| Protein | Number of binned frames, nf | Threshold 1 (T1) |
|---|---|---|
| Respiratory complex I | 5 | 0.5 |
| Trimeric cohesin | 5 | 0.8 |
| NPC | 5 | 0.8 |
| APC/C—affinity chromatography | 3 | 2 |
| APC/C—ion-exchange chromatography | 5 | 3 |
| APC/C—size-exclusion chromatography | 3 | 2 |
| APC/C—crosslinked | 3 | 2 |
| Proteasome | 5 | 3.5 |
