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. 2020 Apr 14;10:6368. doi: 10.1038/s41598-020-62472-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agarose gel electrophoretic analysis of monoplex PCR format for specific detection of B. abortus, B. melitensis, B. ovis, B. suis and B. canis. Lane 1 and 7. 50 bp DNA ladder; Lane 2. B. abortus specific amplicon (1154 bp) with B. abortus specific primer; Lane 3. B. melitensis specific amplicon (745 bp) with B. melitensis specific primer; Lane 4. B. ovis specific amplicon (446 bp) with B. ovis specific primer; Lane 5. B. suis specific amplicon (290 bp) with B. suis specific primer; Lane 6. B. canis specific amplicon (224 bp) with B. canis specific primer; Lane 8. B. abortus rescue amplicon (1478 bp) with primers of the present study except B. abortus specific primer; Lane 9. B. melitensis rescue amplicon (977 bp) with primers of the present study except B. melitensis specific primer; Lane 10. B. ovis rescue amplicon (606 bp) with primers of the present study except B. ovis specific primer; Lane 11. B. suis rescue amplicon (383 bp) with primers of the present study except B. suis specific primer; Lane 12. B. canis rescue amplicon (521 bp) with primers of the present study except B. canis specific primer.