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. 2020 Apr 14;10:6368. doi: 10.1038/s41598-020-62472-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agarose gel electrophoretic analysis of PCR assay using B. abortus, B. melitensis, B. ovis, B. suis and B. canis genomic DNA as template and one pair of primer per reaction. Lane 1 and 7. 50 bp DNA ladder; Lane 2. Specific and rescue amplicon resulted from B. abortus specific primer pair (1154 bp and 1478 bp); Lane 3. Specific and rescue amplicon resulted from B. melitensis specific primer pair (745 bp and 977 bp); Lane 4. Specific and rescue amplicon resulted from B. ovis specific primer pair (446 bp and 606 bp); Lane 5. Specific and rescue amplicon resulted from B. suis specific primer pair (290 bp and 383 bp); Lane 6. Specific and rescue amplicon resulted from B. canis specific primer pair (224 bp and 521 bp).