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. 2020 Apr 14;10:6368. doi: 10.1038/s41598-020-62472-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agarose gel electrophoretic analysis of multiplex PCR assay optimized by incorporation of an internal amplification control (847 bp). All five novel primer pairs were involved in the PCR reaction. Lane 1 and 10. 50 bp DNA ladder; Lane 2 and 9. PCR using B. abortus, B. melitensis, B. ovis, B. suis and B. canis genomic DNA as template DNA; Lane 3. PCR using B. abortus genomic DNA as template DNA; Lane 4. PCR using B. melitensis genomic DNA as template DNA; Lane 5. PCR using B. ovis genomic DNA as template DNA; Lane 6. PCR using B. suis genomic DNA as template DNA; Lane 7. PCR using B. canis genomic DNA as template DNA; Lane 8. PCR without any genomic DNA.