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. 2020 Apr 14;10:6368. doi: 10.1038/s41598-020-62472-3

Figure 6.

Figure 6

Figure illustrating the interpretation of multiplex PCR formats for specific detection of Brucella species. Lane1: Specific PCR amplicons of all five Brucella species are observed for species identification and differentiation; Lane 2: Specific PCR amplicon of 1154 bp indicates presence of B. abortus; Lane 3: Specific PCR amplicon of 745 bp indicates presence of B. melitensis; Lane 4: Specific PCR amplicon of 446 bp indicates presence of B. ovis; Lane 5: Specific PCR amplicon of 290 bp indicates presence of B. suis; Lane 6: Specific PCR amplicon of 224 bp indicates presence of B. canis; Lane 7: Specific PCR amplicons of 1154 bp and 745 bp indicates presence of B. abortus and B. melitensis; Lane 8: Specific PCR amplicons of 1154 bp and 446 bp indicates presence of B. abortus and B. ovis; Lane 9: Specific PCR amplicons of 446 bp and 290 bp indicates presence of B. ovis and B. suis; Lane 10: Specific PCR amplicons of 290 bp and 224 bp indicates presence of B. suis and B. canis; Lane 11: Specific PCR amplicons of 1154 bp, 745 bp and 446 bp indicates presence of B. abortus, B. melitensis and B. ovis; Lane 12: PCR amplicon of 847 bp only indicates that the sample is negative for contamination with any of the five Brucella species.