Table 1.
Bacterial strains used in this study and the resultant PCR amplicon size (bp) with species-specific primer pair in monoplex PCR.
| Reference Strains | Provider | No. of strains (n) | PCR product size (bp) with species-specific primer |
|---|---|---|---|
| B. abortus S19 | IVRI* | 1 | 1154 |
| B. abortus 544 | IVRI | 1 | 1154 |
| B. melitensis 16M | IVRI | 1 | 745 |
| B. ovis ATCC 25840 | NVSL** | 1 | 446 |
| B. suis 1330 | IVRI | 1 | 290 |
| B. suis ATCC 23445 | ATCC*** | 1 | 383 |
| B. canis ATCC 23365 | NVSL | 1 | 224 |
| Pseudomonas aeruginosa ATCC 27853 | ATCC | 1 | No amplification |
| P. azotogenesis NCIM 2075 | NCIMł | 1 | No amplification |
| Species and biovars | Host | ||
| B. abortus biovar 1 | Bovine | 16 | 1154 |
| B. abortus biovar 2 | Bovine | 12 | 1154 |
| B. abortus biovar 3 | Bovine | 9 | 1154 |
| B. abortus biovar 4 | Bovine | 8 | 1154 |
| B. abortus biovar 5 | Bovine | 2 | 1154 |
| B. abortus biovar 6 | Bovine | 4 | 1154 |
| B. abortus biovar 7 | Bovine | 2 | 1154 |
| B. abortus biovar 9 | Bovine | 1 | 1154 |
| B. melitensis biovar 1 | Human | 10 | 745 |
| B. melitensis biovar 2 | Human | 6 | 745 |
| B. melitensis biovar 3 | Human | 3 | 745 |
| B. suis biovar 1 | Human | 2 | 290 |
| B. suis biovar 2 | Human | 2 | 290 |
| B. suis biovar 3 | Swine | 1 | 383 |
| B. suis biovar 4 | Reindeer | 1 | 383 |
| B. suis biovar 5 | Rodent | 1 | 383 |
| Aeromonas hydrophila isolate DFRL1 | Human | 1 | No amplification |
| Proteus mirabilis isolate DFRL1 | Human | 1 | No amplification |
| Citrobacter freundii isolate DFRL1 | Human | 1 | No amplification |
| Yersinia enterocolitica isolate DFRL1 | Human | 1 | No amplification |
*IVRI – Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
**NVSL – National Veterinary Services Laboratories, Ames, Iowa.
***ATCC – American Type Culture Collection, USA.
łNCIM – National Collection of Industrial Microorganisms, Pune, India.