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. 2020 Apr 14;11(4):230. doi: 10.1038/s41419-020-2381-8

Fig. 3. Knockdown of MOB2 promotes the formation of focal adhesions and inhibits anoikis.

Fig. 3

a Gene set enrichment analysis of MOB2-regulated genes and signaling pathway in LN-229 cells, raw data were submitted to NCBI (GSE139339). b Relative mRNA levels in LN-229-shMOB2 cells compared to the control cells (shCON). c, d LN-229 and T98G cells stably knockdowning of MOB2 (c) or SF-539 and SF-767 cells stably overexpressing of MOB2 (d) were stained with p-Paxillin (green) to indicate focal adhesions by immunofluorescence. DAPI was used for nuclear staining. The positive area of the proportion of the total area was quantified. The region enclosed in the red square has been enlarged for a clearer proportion, and the arrows indicated the proportion. Scale bars = 10 or 25 μm. e The capacity of migration and invasion of LN-229 and T98G cells stably knockdowning of MOB2 cells was measured by anoikis assay. All experiments in this figure were performed as three independent experiments. Data are presented as mean ± SEM (**p < 0.01, ***p < 0.001).