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. 2020 Apr 14;11(4):230. doi: 10.1038/s41419-020-2381-8

Fig. 4. FAK-Akt signaling pathway is activated by knocking down MOB2.

Fig. 4

a, b IB with specific antibodies against p-FAK, FAK, p-Akt, Akt, p-Paxillin, Paxillin, and MOB2 in MOB2-depleted LN-229 and T98G cells (a), and SF-539 and SF-767 cells stably overexpressing MOB2 (b). GAPDH was used as a loading control. c LN-229 and T98G cells stably knockdowning of MOB2 or control cells were treated with vehicle (DMSO) or PF566271, IB analysis for p-FAK, FAK, p-Akt, Akt, and MOB2. GAPDH was used as a loading control. d LN-229 and T98G cells stably knockdowning of MOB2 or control cells were treated with vehicle (DMSO) or MK2206, IB analysis for p-FAK, FAK, p-Akt, Akt, and MOB2. GAPDH was used as a loading control. e LN-229 and T98G cells stably knockdowning of MOB2 or control cells were treated the same as in c, the ability of migration and invasion was measured by Transwell chamber assay. f LN-229 and T98G cells stably knockdowning of MOB2 or control cells were treated the same as in d, the ability of migration and invasion was measured by Transwell chamber assay. g SF-539 and SF-767 cells with stable overexpression of MOB2 or control cells were treated with vehicle (DMSO) or SC79, the ability of migration and invasion was measured by Transwell chamber. h LN-229 and T98G cells were transfected with two siFAK (siFAK-1 and siFAK-2) or siControl (siCON) oligonucleotides for 48 h, then IB analysis for FAK, GAPDH was used as a loading control. i LN-229 and T98G cells with a stable knockdown of MOB2 or control cells were transfected with siFAK or siControl (siCON) oligonucleotides for 48 h, then the ability of migration and invasion was measured by Transwell chamber assay. All experiments were performed as three independent experiments. Data were presented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001).