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. 2020 Apr 14;11(4):230. doi: 10.1038/s41419-020-2381-8

Fig. 6. MOB2 is critical for cAMP/PKA-mediated inhibition of cell migration and invasion in glioma cells.

Fig. 6

a LN-229 and T98G cells with a stable knockdown of MOB2 or control cells were treated with vehicle (DMSO) or Forskolin (FSK), IB analysis for p-FAK, FAK, p-Akt, Akt and MOB2, GAPDH was used as a loading control. b SF-539 and SF-767 cells were treated with vehicle (DMSO) or Forskolin (FSK), IB analysis for p-FAK, FAK, p-Akt, Akt, p-CREB and CREB, GAPDH was used as a loading control. c SF-539 and SF-767 cells with stable overexpression of MOB2 or control cells were treated with vehicle (DMSO) or H89, IB analysis for p-FAK, FAK, p-Akt, and Akt, GAPDH was used as a loading control. d LN-229 and T98G cells with a stable knockdown of MOB2 or control cells were treated with vehicle (DMSO) or Forskolin (FSK), and then the ability of migration and invasion was measured by Transwell chamber assay. e SF-539 and SF-767 cells with stable overexpression of MOB2 cells or control cells were treated with vehicle (DMSO) or H89, and then the ability of migration and invasion was measured by Transwell chamber assay. All experiments were performed as three independent experiments. Data are presented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001).