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. 2020 Apr 14;11(4):230. doi: 10.1038/s41419-020-2381-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a LN-229 and T98G cells with a stable knockdown of MOB2 or control cells were treated with vehicle (DMSO) or Forskolin (FSK), IB analysis for p-CREB, CREB and MOB2, GAPDH was used as a loading control. b SF-539 and SF-767 cells with stable overexpression of MOB2 or control cells were treated with vehicle (DMSO) or SQ22536, IB analysis for p-CREB, CREB and MOB2, GAPDH was used as a loading control. c LN-229 and T98G whole-cell lysates (WCL) collected from 10 cm² dishes were subjected to immunoprecipitation (IP) with an anti-MOB2 antibody or an IgG control. d, e 293T cells transfected with GFP-tagged PKA (GFP-PKA) and V5-tagged MOB2 (V5-MOB2). Cell lysates were subjected to immunoprecipitation (IP) with anti-GFP (d) or anti-V5 antibody (e) and immunoblotted (IB) with the indicated antibodies. f Hs683, LN-18 and U87MG cells were treated with vehicle (DMSO) or Forskolin (FSK), IB analysis for MOB1 and MOB2, GAPDH was used as a loading control. g LN-229 and T98G cells were treated with vehicle (DMSO) or H89, IB analysis for MOB1 and MOB2, GAPDH was used as a loading control. All experiments in this figure were performed as three independent experiments.