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. 2020 Apr 14;10:6415. doi: 10.1038/s41598-020-63548-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the experimental treatments with three concentrations (1.5 mM, 15 mM and 30 mM) of nitrogen (¹⁵NH₄⁺, NH₄⁺, ¹⁵NO₃⁻ and NO₃⁻) under normal water (Osmotic potential of the solutions of A₁, B₁, C₁, D₁, E₁ and F₁ was −0.03 Mpa) and drought (Osmotic potential of the solutions of A₂, B₂, C₂, D₂, E₂ and F₂ was −0.1Mpa) conditions were imposed on Kentucky bluegrass ramets (PR, MR and DR). Solutions with ¹⁵NH₄⁺or ¹⁵NO₃^- were added into the black beakers (PR) in each group. Grey beakers holding with MR and DR in the same group were added with either NH₄⁺or NO₃⁻ in the same concentration. PR, MR and DR were connected by rhizome.