FIGURE 4.

Analyses of Mic1 TerL. (A) Phylogenetic tree of TerL proteins from different phages inferred by Neighbor Joining. Colored boxes indicate the characterized clusters which have similar DNA ends. The sequences, except that of Mic1, were classified as described previously (Casjens and Gilcrease, 2009; Huang et al., 2012), and bootstrap analysis was performed with 1,000 repetitions. The scale bar represents 0.2 changes per amino acid. (B) Domain organization of TerL. ATPase domain and nuclease domain are indicated by blue and purple box, respectively. The termini of intein are indicated by orange boxes, and the homing endonuclease domain (HED) in the large intein is indicated by the light gray box. Color blocks indicate the conserved amino acid residues at split sites, the oranges (cysteine, asparagine) for the boundary of intein, the blue (threonine) and purple (cysteine) for the boundary of extein.