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. 2020 Apr 8;10:143. doi: 10.3389/fcimb.2020.00143

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Copyright © 2020 Citro, Bellini, Medda, Sabatini, Tagliabue, Chu and Chiocca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HPV16E6/E7 transduction increases ΔNp63α expression in Human Keratinocytes (HK). HK were transduced with empty or HPV16E6/E7 recombinant retroviral vectors. After selection with G418 cells were harvested. (A) Lysates were collected and analyzed by immunoblotting with the indicated antibodies. (B) Total RNAs were isolated for RT-qPCR. ΔNp63α expression was normalized to RpP0. Results from five independent experiments are expressed as means ± SD of fold changes of ΔNp63α expression of HPV16E6/E7 infected cells over control (empty vector), *P < 0.05 (unpaired t-test).