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. 2020 Apr 8;11:635. doi: 10.3389/fimmu.2020.00635

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Copyright © 2020 Colombo, Bassani, De Angelis, Ruffini, Ottoboni, Comi, Martino and Farina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of iPSC-derived astrocytes. (A) Phase contrast images showing human iNPC (left panel) and human iAstrocytes (right panel). (B,C) Representative immunofluorescence stainings for GFAP, S100β, nestin, vimentin (B) and S1P1, S1P3, IL1R and IL17R (C) in human iAstrocytes. DAPI was used for nuclear staining. (D,E) Percentage of cells positive for astrocyte markers at two timepoints during differentiation. Reported quantifications were performed on three different human iAstrocytes preparations from the same iNPC cell line. Bars represent SEM. Same observations were recorded in human iAstrocytes from a second iNPC cell line. Scale bar = 30 μm.