Figure 6.

Effects of α-cyperone and H₂O₂ on the nuclear translocation of Nrf2 in SH-SY5Y cells. (A) SH-SY5Y cells were treated with α-cyperone (30 μM) for 2 h or H₂O₂ (200 μM) for 24 h, and the nuclear translocation of Nrf2 was determined by immunofluorescence assay. (B–E) After incubation with α-cyperone (30 μM) for various time periods (1-6 h) or with H₂O₂ (200 μM) for 24 h, the nuclear and cytosolic Nrf2 levels were measured by western blot. All experiments were repeated at least three times and similar results were obtained. Representative photomicrographs of Nrf2 are shown. The scale bar represents 100 μ. Data are presented as the mean ± SE, (n= 5 samples per group). ^##p < 0.01 vs. the control group.