Figure 5.

Tomatidine reduces the cell surface expression of the CHIKV E2 protein. Huh7 cells were infected with CHIKV-LR at MOI 1 and treated with 10 µM tomatidine or the equivalent amount of EtOH at the time of infection. (a) Cells were collected, fixed and stained for CHIKV E2 protein on the cell surface at 9 and 16 hpi. (b) Relative fold changes in MFI in the presence of tomatidine compared to the EtOH control at 9 and 16 hpi. Data is represented as mean ± SEM from three independent experiments and differences were assessed with Student’s t-test.