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. 2020 Apr 14;11:1822. doi: 10.1038/s41467-020-15529-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Heat map diagram illustrating the differential expression of miRNAs in islets of HFD compared to normal chow-diet (NCD) mice (a), n = 8 NCD group and n = 7 HFD group), and in Lepr^db/db compared to wide type control mice (b) (n = 8 control group and n = 7 Lepr^db/db group). Red and green indicate increased and decreased gene expression levels, respectively. miR-802 was significantly upregulated in islets of HFD mice (c), and Lepr^db/db mice (d) (n = 7) compared to NCD and wild type control as measured by qRT-PCT. e qRT-PCR was performed to measure the expression level of miR-802 in the islets at different stages (after 0-week, 4-week, 6-week, 8-week and 16-week feeding HFD) during the development of obesity inducing diabetes (n = 5). f qRT-PCR was performed to evaluate the expression levels of miR-802 in different tissues of obese and wild type mice (n = 7). g The expression levels of miR-802 in the serum extracted from lean individuals (n = 22) and overweight individuals (n = 72), using Ce-miR-39-1 as positive control. miR-802 expression was set to 1 in SD. Data sets were statistically analyzed using two-tailed unpaired Student’s t test and Bonferroni Post-hoc correction. h Correlation between miR-802 levels and BMI. Pearson’s correlation coefficients (r) and p values are shown. i The expression level of Pri-miR-802 in the islets of HFD and NCD mice were analyzed by qRT-PCR (n = 3). All experiments above were performed in triplicates, and each group contained three batches of individual samples. The p-values by Pearson’s correlation test h, two-tailed unpaired Student’s t test c, d, g, and i, one-way ANOVA e, or two-way ANOVA f are indicated. Data represent the mean ± SD. Source data are provided as a Source Data file.