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. 2020 Mar 2;52(3):409–422. doi: 10.1038/s12276-020-0390-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The expression level of PROM2 in pancreatic cancer patients treated with gemcitabine. b High expression of PROM2 in pancreatic cancer patients treated with gemcitabine indicates poor overall and disease-free survival (P < 0.001, P < 0.001; TCGA, n = 101). c Representative images (left) and quantification (right) of colonies generated with the indicated cells treated with vehicle or gemcitabine (10 μM). The numbers of clone formation of AsPC-1/vector or Hs 766T/vector has been set for control at 1 (mean ± SD, n = 3; *P < 0.05). d MTT cell viability assay (left) at different concentrations and IC₅₀ value of Gemcitabine (right, 10 μM) in the indicated cells (mean ± SD, n = 3; *P < 0.05). e FACS analysis of Annexin-V and PI staining (left) and quantification (right) of indicated cells treated with Gemcitabine (10 μM) (mean ± SD, n = 3; *P < 0.05).