Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 2;52(3):409–422. doi: 10.1038/s12276-020-0390-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Immunoprecipitation assays showing PROM2 interacts with Akt. b PROM2 binds to Akt endogenously. c Protein expressions of p-BAD and p-Caspase-9 are inhibited when silencing Akt. d IC₅₀ value of gemcitabine in the indicated cells after Akt knockdown (*P < 0.05). e Luminescence signaling of tumors formed by AsPC-1/PROM2 cells after treatment with control or Akt shRNA (*P < 0.05). f Tumor weight of tumors formed by AsPC-1/PROM2 cells after treatment with control or Akt shRNA (*P < 0.05). g Representative images of the immunostaining in tumors tissues (*P < 0.05). h Far-western blotting indicated that Flag-PROM2 interacted with recombinant GST-AKT directly. i Schematic illustration of full length PROM2 and truncations (left) and co-IP assays revealed AKT bound with FL, F1, and F4 fragments, but not with F2 and F3 fragments (right). j Immunoprecipitation assays illustrating the inhibitory effect of PROM2-F4 on the PROM2-AKT interaction. k Quantification of Annexin-V apoptotic cells in indicated cells treated with Gemcitabine (10 μM) (mean ± SD, n = 3; *P < 0.05).