Figure 1.

Gene expression analyses of Tg4–42 + /+ mice. (a) Whole genome sequencing showing the transgene integration site and the deletion of RARB exon 2 on the mouse genome. (b) Binding sites of three different primer pairs used to detect the expression levels of the retinoic acid receptor beta. (c) Gene expression levels measured with conventional qPCR by primer pair 1 in three wild type (WT), three hemizygous (Tg4–42 + /−) and three homozygous Tg4–42 (+/+) striatal samples after cDNA synthesis. HPRT was used as a housekeeping gene to normalize expression levels of the RARB gene. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 3 per group. Mean + SEM. F (2, 6) = 146.6, R² = 0.9794. Significances are labelled as followed: ***p < 0.001 against WT and ^### as indicated). (d) qPCR results of three wild type (WT) and three homozygous Tg4–42 (+/+) striatal samples using primer pair 1, 2 and 3. Mean Cq values of RARB were normalized with mean Cq values of HPRT.