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. 2020 Apr 14;10:6313. doi: 10.1038/s41598-020-63059-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization and transcriptional activation analysis of OsNPR3.3. (a) Nuclear localization of OsNPR3.3. Protoplasts were transfected with the OsNPR3.3-YFP construct. OsABF1-RFP was used as a nuclear localization marker. The YFP (left) and RFP (right) signals in the top panel are green and red, respectively. The bright field (left) and merged (right) images are shown at the bottom. The yellow signal shows that the YFP and RFP overlapped very well in the nucleus. (b) Transcriptional activation test of OsNPR3.3 in yeast cells. OsNPR1 was used as a positive control. All the yeast cells were 10-fold serially diluted and spread on culture medium. BD is the DNA binding domain of the yeast GAL4 transcription factor.