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. 2020 Apr 14;10:6336. doi: 10.1038/s41598-020-63297-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results obtained after all-out 30 s (AO30 recovery) at tethered running in a non-motorized treadmill (mean ± SEM at end and each 2 min during passive recovery, n = 12) (A) Blood lactate concentration (mM), (B) Heart rate (bpm), (C) arterial oxygen saturation – SpO₂ (%), (D) Tissue saturation index (TSI) (%) in biceps brachii (BB) and vastus lateralis (VL), (E) Changes (ΔµM) in oxyhemoglobin ([O₂Hb]) occurred in BB and VL, (F) Changes (ΔµM) in deoxyhemoglobin ([HHb]) in BB and VL, and (G) Changes (ΔµM) in total hemoglobin ([tHb]) occurred in BB and VL, measured by near infrared-spectroscopy (NIRS). *Indicates the difference between responses obtained in BB and VL at the same moment (P ≤ 0.05).