Fig. 7. Prazosin overcomes sorafenib resistance in vivo.
HuH-7R cells were subcutaneously injected into nude mice. At a tumor volume of ~200 cm3, mice were orally treated with vehicle (N = 5), sorafenib at 30 mg/kg (N = 5), and/or prazosin at 3 mg/kg (N = 5) for 18 days. a Measurement of tumor volumes. b Upper, representative images of tumor xenografts. Lower, tumor weights after the end point. c Tumor sections were analyzed via immunohistochemistry. Paraffin-embedded sections of tumor tissue were stained with the indicated antibodies. Representative sections at ×400 magnification. d Survival times were calculated with the Kaplan–Meier method. Statistical data were calculated from five replicates under a single independent experimental design (ns not significant; **p < 0.01).