Fig. 7. IFN-γ positively regulates MHC II expression of ILC3s.

a Normalized expression of Ciita mRNA isoforms regulated by promoters pI (n = 5 distinct SP ILC3 samples and n = 9 distinct SI ILC3 samples), pIII (n = 5 distinct SP ILC3 samples and n = 9 distinct SI ILC3 samples), and pIV (n = 5 distinct SP ILC3 samples and n = 8 distinct SI ILC3 samples) in SP and SI ILC3s. ILC3s were sorted as depicted in Supplementary Fig. 2a. Five independent experiments. b Expression of IFN-γ in the SP and the SI of WT mice. n = 5 mice of two independent experiments. c MHC II expression of SI ILC3s from WT (n = 6 distinct samples) and Ifng^−/− (n = 6 distinct samples) mice. NCR⁻ ILC3s were gated as shown in Fig. 1. Two independent experiments. d MHC II expression of SI ILC3s (Rag2^−/−) stimulated for 48 h (n = 9 distinct samples of three independent experiments) or 120 h (n = 10 (w/o) distinct samples and n = 11 (IFN-γ stimulated) distinct samples of four independent experiments) with or without (w/o) IFN-γ. ILC3s were sorted as depicted in Supplementary Fig. 2a. e Normalized expression of Ciita mRNA isoform regulated by promoter pIV in SI ILC3s stimulated with or w/o IFN-γ for 120 h. n = 5 distinct samples of five independent experiments. ILC3s were sorted as depicted in Supplementary Fig. 2a. f SI ILC3s (sorted from Rag2^−/− as depicted in Supplementary Fig. 2a) were stimulated for 120 h with or w/o IFN-γ. Naive CFSE-labeled OT-II^tg CD4⁺ T cells were cultured with 1 × 10⁴ IFN-γ stimulated or unstimulated ILC3s in the presence of Ova peptide. n = 7 distinct samples of three independent experiments. Each symbol represents a sample and the bar graph represents the mean ± s.e.m. n.s., not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001, calculated with two-tailed unpaired Student’s t test (a Ciita pI and pIII, b, c, and e), Mann–Whitney test (a Ciita pIV) or with mixed-effects models (two-sided) using lmerTest (d and f). Source data are provided as a Source Data File.