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. 2020 Apr 14;10:6323. doi: 10.1038/s41598-020-62953-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Snol-A, but not Snin-I, caused potent cytotoxicity to cancer cells. (A,B) Viability of control, Snol-A (A) and Snin-I (B) treated (48 h) cancer cells (SKOV-3, U2OS, Saos-2 and MDA-MB-231). Snol-A, but not Snin-I, showed dose-dependent toxicity. (C) Phase contrast images of control and treated SKOV-3 and U2OS cells; Snol-A, not Snin-I, treated cells showed stressed morphology marked by flat, irregular and branched phenotypes. (D) Colony-forming assay showing crystal violet stained colonies in control and Snol-A treated U2OS, SKOV-3, Saos-2 and MDA-MB-231 cancer cells. Quantitation of colony forming assay is shown on the right.