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. 2020 Apr 14;10:6323. doi: 10.1038/s41598-020-62953-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CARF-targeting by Snol-A reduced cancer cell migration and invasion. (A) Phase-contrast images taken from 0, 24 and 48 h time-points in control and Snol-A treated SKOV-3 cells, showing inhibition of cell migration with 0.5, 2 and 4 μM Snol-A does in wound-healing assay. Quantitation of scratched area (%) in these cells is shown at the right. (B) Matrigel invasion assay showing Crystal violet stained invaded cell counts in control, 0.5 and 2 μM Snol-A treated SKOV-3 cells. Quantitation of the percent invasiveness, calculated based on cell count is shown below. (C) Immunoblots showing decrease in CARF protein in 2 and 4 μM Snol-A treated cells that was accompanied by decreased level of the Vimentin, Smad2/3, hnRNPK and MMP-9, key cell migration markers involved in cancer metastasis. Quantitation of their levels is shown on the right. (D) Immunostaining showing decrease in nuclear β-catenin in 2 and 4 μM Snol-A treated SKOV-3 cells, as compared to the untreated control. Decrease in Vimentin, Fibronectin and hnRNP-K was also evident in 2 and 4 μM Snol-A treated SKOV-3 cells. Quantitation of fluorescent intensities is shown on the right.