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. 2020 Apr 14;11:1805. doi: 10.1038/s41467-020-15571-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematized depiction of the ES2 lncRNA and the segmented transcripts. Apparent binding affinity displayed as the average and s.e.m. of n = 6 technical replicates excluding 1–354 ES2, which is n = 10 technical replicates). b Sfold⁴⁴ predicted secondary structure of ES2, nts 276–354, and minimized loop constructs. c Average binding curve for each Sox2-RNA interaction with error bars displaying the standard deviation of each measurements. K_D,app is reported for the first transition and presented as the average and the s.e.m. of n = 6 technical replicates.