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. 2020 Apr 14;10:6358. doi: 10.1038/s41598-020-63031-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surgical procedures. Late pregnant or newly parturient pregnant rats were anesthetized (A) and a modified speculum (B) was placed to visualize and generate laceration of the posterior vaginal wall extending from the posterior perineum to just below the cervix (C).