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. 2020 Apr 14;10:6358. doi: 10.1038/s41598-020-63031-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pregnancy and normal parturition on vaginal FBLN5. (A) Representative immunoblot (30 µg urea-extracted protein per lane) of vaginal muscularis from nonpregnant controls and late pregnant animals. The >75 kDa protein is nonspecific interaction of protein with primary antibody. Specific FBLN5 immunoreactive proteins are noted by brackets with proteins of lower molecular weight likely representing differences in glycosylation or splice variants. Bands within brackets were combined for quantification. (B) Quantification of FBLN5 per mg protein in vaginal muscularis from nonpregnant (n = 6, NP CTL) and late pregnant (n = 13) rats. *P < 0.01, Student’s t test. (C) Effect of pregnancy and parturition without injury on FBLN5 content of the rat vaginal wall. Data represent mean ± SEM of relative FBLN5/mg protein in 4 animals per group. *One-way analysis of variance (P < 0.05). (D) Effect of simulated obstetrical injury on recovery of FBLN5 content in the postpartum vaginal wall. Arrow denotes time of injury as described in Materials and Methods. **significant differences relative to noninjured time point in Panel C. Data represent mean ± SEM of 4 animals in each group.