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. 2020 Apr 14;10:6358. doi: 10.1038/s41598-020-63031-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in (A) injury + PBS (Inj 3d) and injury + actinonin (Inj + Act 3d). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon E1600 microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents (B) elastin fiber length (µm) and (C) percent area covered by elastic fibers in pregnant (D21) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P < 0.05 compared with injury alone time point, t-test.