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. 2020 Apr 14;10:6358. doi: 10.1038/s41598-020-63031-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative histologic images of nonpregnant, day 21 and postpartum vaginal wall after injury. Microscopic images of vaginal muscularis and epithelium in nonpregnant (NP) (A), day 21 (B), 3d after delivery in injury + PBS (C) and injury + actinonin (D). The 4–5 µm sections of full-thickness vaginal rings were stained with Masson trichrome. Quantitative analysis of area of injury (outlined with yellow dashed line. Vaginal rings in the actinonin treated specimen (D) are intact with statistically smaller area of injury. Injury + PBS specimens (C) maintain persistent defects at injury site. (E) Area of injury (mm²) for postpartum injured animals treated with PBS (black) or actinonin (red). PBS, phosphate buffered saline; Act, actinonin. 4X magnification, Scale bar, 500 µm.