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. 2020 Apr 14;10:6358. doi: 10.1038/s41598-020-63031-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of Collagen Types I and III in the vaginal wall of pregnant and nonpregnant rats. Immunofluorescence of Collagen I (LEFT) and III (RIGHT) in uninjured rat vaginal wall in nonpregnant virginal rats (top row) and pregnant rats on day 21 of gestation (bottom row). Sections of full-thickness vaginal rings (4–5 µm) were labeled with primary antibodies to collagen I and III. Green = collagen I, Red = Collagen III, Blue = DAPI. Increased ratio of collagen I/III noted in each specimen. Increased content of both collagen subtypes noted in pregnant specimens. Scale bars 50 µm. Graphical depiction (Right) of collagen area between nonpregnant controls (NP) and pregnant (Preg D21) animals. mus, vaginal muscularis; epi, epithelium; bv, blood vessel.