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. 2020 Apr 14;10:6396. doi: 10.1038/s41598-020-63357-1

Figure 3.

Figure 3

LXR are transcriptionally active in DRG. (A) In situ hybridization assay confirming presence of LXRa and LXRb mRNA in mouse DRG (scale 50 µm) (green-LXRα/LXRβ; blue-nuclei). (B) Luminescence dose-response curve for LXR agonist, GW3965, in DRG primary neuronal cultures transduced with pGreenFire1‐LXRE in LXRα Lentivector (n = 3 individual experiments). (C) GFP expression of GW3965 treated DRG primary neurons transduced with pGreenFire1‐LXRE in LXRα Lentivector using fluorescence imaging and flow cytometry (n = 3 individual experiments) (scale 100 µm). (D) Activation of LXR by GW3965 increases gene expression of LXR canonical pathway in the DRG (vehicle group treated as 100%) (n = 3 experiments in triplicate). (E) GW3965 mediated-luciferase activity in DRG primary neuronal cultures transduced with pGreenFire1‐LXRE in LXRα Lentivector treated with or without palmitate (n = 3 experiments in triplicate). All data are Mean±S.E.M. *p < 0.05; ***p < 0.0005.