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. 2020 Apr 14;10:6353. doi: 10.1038/s41598-020-63122-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OPLS-DA model analysis and DEMs identification. (A–D) are OPLS-DA model analysis results (A,B) data from positive ion mode; (C,D), negative ion mode) based on LC/MS data of metabolites from Pacific abalone hepatopancreas samples of control (blue) and heat shock groups (red). Permutation test confirmed the validation of each model (E–H). (I,J) show Venn diagram analysis results of DEMs identified from different comparison pairs. L, Pacific abalone juveniles acclimated for 62 days at 10 °C and then cultured for 7 days at 20 °C; LH, Pacific abalone juveniles from L group treated 3 hours by 31 °C heat shock; H, Pacific abalone juveniles acclimated 62 days at 30 °C and then recovered after 7 days at 20 °C; HH, Pacific abalone juveniles from H group treated for 3 hours with 31 °C heat shock. Image was created by R software version 3.5.3 (https://www.r-project.org/).