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. 2020 Apr 14;11:1833. doi: 10.1038/s41467-020-15290-0

Fig. 2. NHWD-870 inhibited BRD4 phosphorylation and c-MYC expression, and suppressed cell growth.

Fig. 2

a Representative western blot analysis of p-BRD4 (s484/488), BRD4, c-MYC, and GAPDH after treatment of MDA-MB231, SUM159, A2780 ES-2, and H526 cells with JQ-1 or NHWD-870 at the indicated concentrations for 24 h. GAPDH served as the loading control. Selected from three independent experiments. b MTT assays of H526, A2780, ES-2, and MDA-MB231 cells treated with JQ-1 or NHWD-870 at the indicated concentrations for 5 days. Data are presented as mean ± SEM from three independent experiments. c, d Clonogenic assays of H526, A2780, ES-2 and MDA-MB231 cells treated with 250 nM JQ-1 or NHWD-870 for 5 days. Shown are representative images (c) and quantification of colonies (d). Data are presented as mean ± SEM from five independent experiments. e, f 3D matrigel assays of H526, A2780, ES-2, and MDA-MB231 cells treated with 100 nM JQ1 or NHWD-870. Shown are representative images (e) and quantification of relative colony sizes (f). Scale bar, 50 μm. Data are presented as mean ± SEM from five independent experiments. p values were calculated using two-tailed, unpaired t tests in this figure. **p < 0.01; ***p < 0.001. Source data are provided as a Source Data file.