Table 1.
Wild type and CL-lacking cells are able to maintain a WT level ΔμH+ at two different pH values of the growth medium.
| W3110 | BKT12 | ||
|---|---|---|---|
| ΔΨ | pH 5.5 | −113 ± 8 | −117 ± 6 |
| pH 7.0 | −98 ± 6 | −95 ± 6 | |
| ΔpH | pH 5.5 | −75 ± 7 | −70 ± 9 |
| pH 7.0 | −35 ± 6 | −38 ± 8 | |
ΔΨ (mV) and ΔpH (mV) were determined from radioactive lipophilic cation TPP+ and weak acid DMO distribution between the internal volume of intact cells and the cell medium by the silicone oil-spin method. Values are means (± S.D.) from three experiments, where each determination was done in duplicate.