Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 14;10:6272. doi: 10.1038/s41598-020-62925-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The naïve mESCs differentiated into cortical progenitors in 5 days in monolayer culture. The tile and zoomed images show that the differentiated neural progenitors, labelled with (A,A′) DAPI, widely express the neural progenitor marker (B,B′) Nestin and the forebrain/midbrain marker (C,C′) Otx2. (D,D′) Merged images. The lower panel of the photomicrographs show that neural progenitors stained with (E,E′) DAPI, highly express the early cortical marker (F,F′) Pax6 and the forebrain/midbrain marker (G,G′) Otx2. (H,H′) Merged images. Flow cytometry comparative analysis between mESCs (control) and neural progenitors on day 5 depict (I,J) dramatic elevation in Nestin expression, (K,L) disappearance of Oct4, emergence of both (M,N) Otx2 and (O,P) Pax6 in cortical progenitors, all of which when compared to their negative counterparts. (Q,R) The null expression FoxA2 in both cortical progenitors and their negative matches. Log-2-fold change in the transcriptional expression of (S) Nestin, (T) Oct4, (U) Otx2, (V) Pax6, (W) Foxg1, and (X) FoxA1 supporting the findings obtained from the flow cytometry analyses. Data represents mean ± SEM, n = 5, Students t-test ***p < 0.001. Scale bars = 100 um.