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. 2020 Apr 14;10:6391. doi: 10.1038/s41598-020-63318-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRP predominantly recognizes the edges or specific faces of MSU crystals. (a) MSU crystals (lot 2) were incubated for 30 min at 37 °C with human serum (CRP 0.2 µg/ml) with 30 µg/ml recombinant CRP added. CRP binding was analyzed using CRP antibody and anti-rabbit-AlexaFluor488. AlexaFluor488-fluorescence of the crystals was detected by fluorescence microscopy; scale bar = 40 µm. (b) MSU crystals (lot 2) were incubated as described in a in 30 µg/ml CRP-containing HBSS 5% BSA with 30 µg/ml recombinant CRP added. Microscopic detection of bound CRP performed as in a. (c) t-CPPD (self-made) was incubated in HBSS 5% BSA with 30 µg/ml recombinant CRP added as described in b. Microscopic detection of bound CRP performed as in a. Each experiment is representative of at least 2 independent experiments.