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. 2020 Apr 14;10:6391. doi: 10.1038/s41598-020-63318-8

Figure 5.

Figure 5

Co-localization of CRP and C3 on opsonized MSU crystals. (a) Confocal microscopy of MSU crystals (lot 1), which were incubated for 30 min at 37 °C with human serum (CRP 0.3 µg/ml) with or without addition of 40 µg/ml purified CRP, washed extensively and stained with rabbit anti-CRP plus anti rabbit AF568 (red) and mouse anti-C3/C3b plus anti mouse AF488 (green). DIC = digital interference contrast; scale-bar = 40 µm. (b) MSU crystals lot 2 were incubated in human serum (CRP 0.6 µg/ml) with or without addition of 40 µg/ml purified CRP, stained and microscopically detected as in a.