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. 2020 Apr 14;11:1781. doi: 10.1038/s41467-020-15609-x

Fig. 4. Nuclease contamination cannot explain RNA–DNA hybrid formation by PRC2.

Fig. 4

a Scheme of experiment to test whether PRC2 generate a long ssDNA filament. Linear DNA was incubated with PRC2 or exonuclease III and purified. SSB protein was added to pre-treated DNA and samples were visualized by electron microscopy. b, c Representative EM pictures of DNA pre-treated with Exonuclease III (b) or with PRC2 (c). Arrows indicate DNA with (b) or without (c) SSB coating. d Scheme of experiment to test whether pre-incubation of DNA with PRC2 allows RNA–DNA hybrid formation in the absence of PRC2.is sufficient. e, f Representative gel (e) and quantification (f) of strand exchange reactions using DNA that was pre-treated with PRC2 (400 nM) as the substrate. n = 3. Graph shows the mean +/− S.E.M. Source data are provided in Source Data file.