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. 2020 Apr 14;11:1791. doi: 10.1038/s41467-020-15628-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a cdbA-B locus. Start and stop codons are indicated. +1 indicates first nucleotide in the cdbA start codon. Arrows indicate direction of transcription. MXAN locus tags are indicated for the genes flanking cdbA-B. See Supplementary Fig. 1 for annotation of these genes. b CdbA and CdbB binding to ³²P-c-di-GMP in DRaCALA in the absence and presence of unlabeled c-di-GMP and GTP. Similar results were obtained in two independent experiments. Source data are provided as a Source Data file. c ITC of c-di-GMP-binding to CdbA variants. In each experiment, the original titration traces (top panel) and integrated data (bottom panel) are shown. Solid line in the bottom panel represents the fit of the integrated titration peaks to a one-site binding model. Dissociation constants (K_D) and thermodynamic parameters are listed for CdbA^WT for which binding was detected. d Analytical SEC of CdbA variants in the presence and absence of c-di-GMP. Arrows indicate elution volume and mass of molecular weight standards. n = 5 for CdbA^WT, n = 2 for CdbA^K8A/S10A and CdbA^R27A/R30A. Table indicates calculated and apparent molecular mass of CdbA variants. Source data are provided as a Source Data file.