Table 1.
Characteristics of each vessel type classified in this study.
| Vessel types classified in this study | |||
|---|---|---|---|
| Artery | Arteriole | Sinusoid | |
| Vascular markers | |||
| CD31 (IHC, FCM) | + | + | + |
| VE-cadherin (FCM) | + | + | + |
| AF633 (IVI, IHC) | + | − | − |
| α-SMA (IHC) | + | − | − |
| NG2 (IHC) | + | + | Low/− |
| Sca-1 (IVI, IHC, FCM) | + | + | Low |
| AcLDL (IVI, FCM) | − | − | + |
| Podoplanin (FCM) | − | − | + |
| Vessel typesthat are considered identical in previous reports | |||
| Bixel et al., Cell Reports, 2017 (Skull bone) | Arterial vessel | Arterial vessel | Post-arterial / Intermediate / Sinusoidal capillaries |
| Itkin et al., Nature, 2016 (Long bone) | Artery | Endosteal arterioles | Sinusoid |
| Acar et al., Nature, 2015 (Long bone) | Arteriole | TZ vessel | Sinusoid |
| Kusumbe et al., Nature, 2015 (Long bone) | Artery | Arteriole | Type H/L capillary |
| Dimensions and hemodynamic parameters | |||
| Diameter (μm) |
10.9 ± 4.6 (6.9–17.2) |
6.3 ± 1.2 (4.7–8.6) |
20.8 ± 5.2 (14.0–28.1) |
| Velocity (mm/s) |
2.0 ± 0.9 (1.1–3.1) |
2.1 ± 1.4 (1.5–5.5) |
0.2 ± 0.1 (0.1–0.3) |
| RBC flux (pL/s) |
59.6 ± 82.7 (12.8–183.4) |
14.9 ± 13.5 (3.5–44.9) |
17.9 ± 11.6 (1.7–34.3) |
| RBC density (%) |
43.2 ± 10.4 (28.1–51.1) |
39.0 ± 11.8 (19.6–53.3) |
44.8 ± 6.9 (29.5–50.7) |
| Viscosity (cP) |
2.5 ± 0.3 (2.0–2.8) |
2.4 ± 0.4 (1.8–2.8) |
2.5 ± 0.2 (2.1–2.7) |
| Shear rate (1/ms) |
1.52 ± 0.60 (0.79–2.24) |
2.68 ± 1.42 (1.64–6.08) |
0.07 ± 0.03 (0.03–0.11) |
| Shear stress (dyn/cm2) |
37.1 ± 11.7 (20.0–45.9) |
63.6 ± 35.0 (37.4–144.7) |
1.9 ± 0.9 (0.7–3.2) |
| NE reactivity | |||
| % Change of Diameter (%) |
−80.5 ± 73.0 * (−189.1– −36.2) |
2.6 ± 14.5 (−21.0–24.0) |
8.0 ± 11.0 (−8.4–25.6) |
The combinations of vascular markers that were used to classify BM blood vessels during immunohistochemical (IHC), flow cytometric (FCM) and in vivo imaging (IVI) analysis in this study. Numbers are means ± SD, ranges are shown in parentheses. Asterisk indicates p < 0.05 (PBS vs. NE, paired t-test). These data obtained from four arteries, eight arterioles and eight sinusoids in five mice.