Table 1.
Gene | Primer sequence |
---|---|
NEAT1 (human) | F: 5′-TTTGTGCTTGGAACCTTGCT-3′ |
R: 5′-TCAACGCCCCAAGTTATTTC-3′ | |
LATS2 (human) | F: 5′-ACTTTTCCTGCCACGACTTATTC-3′ |
R: 5′-GATGGCTGTTTTAACCCCTCA-3′ | |
NEAT1 (rat) | F: 5′-ACTGCTTGACACCCCATGCC-3′ |
R: 5′-CGGTGATGACCACGGCTACC-3′ | |
LATS2 (rat) | F: 5′-CCAACAGCAAGCACCCAGAG-3′ |
R: 5′-CGACCGCACCTCCTAACTC-3′ | |
β-Actin (human) | F: 5′-CCAAGGCCAACCGCGAGAAGATGAC-3′ |
R: 5′-AGGGTACATGGTGGTGCCGCCAGAC-3′ | |
β-Actin (rat) | F: 5′-AGAGCTACGAGCTGCCTGAC-3′ |
R: 5′-AGTACTTGCGCTCAGGAGGA-3′ |
RT-qPCR reverse transcription quantitative polymerase chain reaction, NEAT1 nuclear-enriched abundant transcript 1, LATS2 large tumor-suppressor kinase 2, F forward, R reverse.