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. 2020 Mar 10;52(3):461–472. doi: 10.1038/s12276-020-0387-z

Table 1.

Primer sequences for RT-qPCR.

Gene Primer sequence
NEAT1 (human) F: 5′-TTTGTGCTTGGAACCTTGCT-3′
R: 5′-TCAACGCCCCAAGTTATTTC-3′
LATS2 (human) F: 5′-ACTTTTCCTGCCACGACTTATTC-3′
R: 5′-GATGGCTGTTTTAACCCCTCA-3′
NEAT1 (rat) F: 5′-ACTGCTTGACACCCCATGCC-3′
R: 5′-CGGTGATGACCACGGCTACC-3′
LATS2 (rat) F: 5′-CCAACAGCAAGCACCCAGAG-3′
R: 5′-CGACCGCACCTCCTAACTC-3′
β-Actin (human) F: 5′-CCAAGGCCAACCGCGAGAAGATGAC-3′
R: 5′-AGGGTACATGGTGGTGCCGCCAGAC-3′
β-Actin (rat) F: 5′-AGAGCTACGAGCTGCCTGAC-3′
R: 5′-AGTACTTGCGCTCAGGAGGA-3′

RT-qPCR reverse transcription quantitative polymerase chain reaction, NEAT1 nuclear-enriched abundant transcript 1, LATS2 large tumor-suppressor kinase 2, F forward, R reverse.