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. 2020 Mar 10;52(3):450–460. doi: 10.1038/s12276-020-0396-y

Fig. 3. Knockdown of TGF-β receptor I and II blocks TGF-β1-induced VEGF expression and secretion.

Fig. 3

a, b SVOG cells were transfected with 50 nM control siRNA (si-Ctrl), TGF-β receptor I siRNA (si-TβRI) (a) or TGF-β receptor II siRNA (si-TβRII) (b), and after 48 h they were treated with 5 ng/mL TGF-β1 for 6 h. VEGF, TβRI, and TβRII mRNA levels were examined by RT-qPCR. c, d SVOG cells were transfected with 50 nM control siRNA (si-Ctrl), TGF-β receptor I siRNA (si-TβRI) (c) or TGF-β receptor II siRNA (si-TβRII) (d), and after 48 h they were treated with 5 ng/mL TGF-β1 for 24 h. Protein levels of VEGF in culture media were examined by ELISA. The results are expressed as the mean ± SEM of at least three independent experiments. The values without a common letter are significantly different (p < 0.05).