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. 2020 Mar 10;52(3):450–460. doi: 10.1038/s12276-020-0396-y

Fig. 5. ERK1/2 and p38 MAPK signaling pathways are involved in TGF-β1-induced VEGF expression and secretion.

Fig. 5

a SVOG cells were treated with 5 ng/mL TGF-β1 for 10 min. Phosphorylated and total ERK1/2 and p38 expression levels were determined by western blot. b SVOG cells were pretreated with a vehicle control (DMSO), 10 µM U0126 or 10 µM SB203580 for 1 h, and then they were treated with 5 ng/mL TGF-β1 for 6 h. VEGF mRNA levels were examined by RT-qPCR. c SVOG cells were pretreated with a vehicle control (DMSO), 10 µM U0126 or 10 µM SB203580 for 1 h, and then they were treated with 5 ng/mL TGF-β1 for 24 h. VEGF protein levels in culture media were examined by ELISA. The results are expressed as the mean ± SEM of at least three independent experiments. The values without a common letter are significantly different (p < 0.05).