Fig. 5. Treatment with ONC201/TIC10 and 2-Deoxyglucose inhibits tumour formation on the CAM.

a–f 1 × 10⁶ ULM-GBM-PC128 or 2 × 10⁶ U251 cells were seeded on each CAM of fertilised chicken eggs. After 24 h, the cells were treated twice daily with 50 µM ONC201/TIC10, 25 mM 2-Deoxyglucose or both compounds. On day 5, the tumours were harvested, and photographs were taken prior to fixation in 10% formalin and histological processing. a Representative photographs of ULM-GBM-PC128 tumours located on the CAM at day 5. The circle has a diameter of 5 mm. b Representative histological images of tumours on the CAM stained with haematoxylin and eosin (magnification, ×4; scale bar = 500 µm). c, d Photographs of tumours treated as indicated. Three to five technical replicates are shown for each treatment group. e, f Quantitative representation of the tumour area as assessed by Image J (NIH, Bethesda, MD; http://imagej.nih.gov/ij). Data are presented as mean of at least three tumours per treatment group and standard deviation (SD).