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Validation of promoter DNA methylation status by pyrosequencing. The methylation levels of candidate marker genes analyzed by Infinium for the 40 training samples are shown on the color scale (top left). Validation by quantitative methylation analysis was performed using pyrosequencing. Pyrosequencing primers for 8 of the 10 markers could be generated (bottom left). Receiver operating characteristic curves were drawn using Infinium and pyrosequencing data of the 8 markers (right)
