Figure 2.

Cisplatin‐induced programmed death‐ligand 2 (PD‐L2) expression is mediated by signal transducers and activator of transcription 1/3 (STAT1/3) activation in HSC‐2 cells. Expression of PD‐L2, STAT1 and STAT3 in HSC‐2 cells with or without cisplatin treatment (2 μmol/L) for 0‐48 h using quantitative RT‐PCR analysis (A) and western blot analysis (B). Bar graphs show the mean from seven culture wells (mean ± SEM). * and ** indicate P < 0.05 and P < 0.01, respectively, vs control (0 h). The effect of a STAT1 or STAT3 inhibitor on PD‐L2 expression (C) and the activation of STAT1/3 phosphorylation (D). HSC‐2 cells were incubated with cisplatin in the presence or absence of a STAT1 or STAT3 inhibitor for 24 h. Similar results were obtained from three independent experiments